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In other words, the post-translational maturation appears to be hampered in the mutant SOD1 proteins, resulting in an increased propensity of SOD1 to misfold into oligomers and aggregates

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To generate baculovirus from the pAC8-derived constructs, 1 g plasmids were co-transfected with linearized baculoviral DNA in Spodoptera frugiperda Sf9 cells grown in ESF 921 medium (Expression Systems) at a density of 1.0 10 6 cells/ml